CRISPR-Cas9 is a groundbreaking gene-editing technology that allows scientists to precisely modify specific genes within the DNA of living cells. The system uses the Cas9 enzyme, which acts like molecular scissors to cut DNA, guided accurately to its target by a specially designed guide RNA.

This powerful tool is transforming biomedical research and opens up new possibilities for understanding and treating diseases, including cancer.

During this minor, you will gain hands-on experience with CRISPR-Cas9 by using it to knock out the Nrf2 gene. This gene encodes a transcription factor that plays a crucial role in cellular stress responses and is dysregulated in certain types of cancer. By disabling Nrf2, you will explore how gene function can be studied and how gene-editing technology contributes to innovative cancer research.

Minor content 

During the Cell and Gene Therapy minor, you will apply CRISPR-Cas9 technology to knock out the Nrf2 gene. This gene encodes a transcription factor that is dysregulated in certain types of cancer, making it highly relevant in oncological research. A human cancer cell line is available to investigate the biological consequences of this CRISPR-Cas9–mediated knockout.

Throughout the project, you will work extensively with molecular biology and cloning techniques to successfully design and execute your research.

From design to discovery

You will begin by designing your own CRISPR-Cas9 experiment. Using in silico tools, you will develop your gene-editing strategy and carefully plan your approach. In parallel, you will participate in laboratory training sessions to master the molecular and cloning techniques required for your project.

Once your project proposal has been approved, you will have the freedom in the lab to independently organize and carry out your research. Weekly progress meetings provide a structured moment to discuss results, troubleshoot challenges, and ask questions.

Strong theoretical foundation

In addition to the practical component, the minor includes two theoretical modules in which we explore various cloning strategies and molecular tools commonly used in gene-editing workflows, as well as advanced cell biology and immunology. These techniques are discussed within the broader biological context of NRF2 and its role in cancer development and progression.

This minor offers a unique combination of independent research, technical skill development, and in-depth scientific understanding — preparing you for advanced work in biomedical research and biotechnology.

Structure of the minor

The minor consists of:

•  A research project (24 EC)

• Flanking Theory (2 x 3 EC)

The following working methods are offered within the project:
• Introductory guest lecture by the client
• Interim contact moments with the client
• Work discussions with fellow students
• Literature research and presenting it to fellow students
• Writing an Action Plan
• Independently carry out the research project in the laboratory
• Reporting the research results in an article
• Participation in a poster symposium

The minor Precision DNA Editing: CRISPR & Cancer Therapy is interesting for students with a biological background who are interested in the medical field and who have a VMT certificate. Studies that match the minor include Biology and Medical Laboratory Research, Biotechnology.

Assignments in this minor are:
- two theoretical exams
- a research proposal
- an oral presentation
- a research paper
- a poster presentation

You will also receive feedback on your professional skills and development.