Kies op maat

Inloggen Menu

Bioengineering and - omics

The CRISPR Cas9 technique enables you to change genetic material in a way that some people describe as copy and paste. Apart from being exiting this technique has several great possibilities and unquestionable benefits. Like editing immune cells for cancer therapy, improving IVF (in vitro fertilization), creating seedless tomatoes or creating biofuel. Genetic manipulation of microorganisms, plants and mammalian cells can be used to let these cells produce new molecules and/or knocking out genes encoding for proteins and allowing thus to obtain knowledge about these proteins and consequent metabolic pathways. The holistic approach of –omics (genomics, transcriptomics and metabolomics) allows to shed a light on the consequences of such genetic changes.

This minor focuses on learning and applying the technique of CRISPR Cas9 and on exploring its consequences using –omics approach. Students work on real-world project: knocking-out gene in mammalian cells in order to study possible target for cancer therapy, such contributing to sustainable development goal of Good health and well-being. This project is developed in cooperation with the international partner.

In this minor cloning strategy will be first designed and further carried out in the laboratory. Students will analyse the consequences of the genetic manipulation and perform functional studies of obtained cells. This course is intended to mimic the practice of a research group. Students are responsible for their own research and report the results by weekly meetings/presentations to the rest of the research groups (students) and their group leaders (lecturers). Additionally, students will discuss with their the class mates current findings in the bioengineering and –omics field via presenting selected research articles during -omics journal club and they will receive guest (international) lectures about their research topic and about legislation and ethics of GMO. During the minor students will also go for an (international) excursion and visit biotechnological company. As a closing moment of this course, students will present their results at self-organized congress.

Added value

Following this course gives students exceptional opportunity to experience the newest techniques of molecular cloning. Students will obtain the expertise of independent design and execution of molecular cloning experiments, big data interpretation and reporting. They will also experience working in a research group as biotechnology analysts. It gives them advantage when job requests are focused on molecular cloning techniques using CRISPR Cas9 technology. Investigating molecular cloning and its influence on the metabolism of the cell will deepen their knowledge in molecular biology.

Leerdoelen

- Students will be able to orientate in the field of genetic engineering.

- Students will be able to perform predesigned CRISPR Cas9 experiment and design their own CRISPR Cas9 gene editing project.

- Students will be able to set up a research proposal and execute the practical part in the laboratory.

- Students will be able to use cloning software and online tools to visualize cloning strategy.

- Students will be able to present the cloning design/research proposal.

- Students will be able to interpret and report data obtained from molecular cloning and software tools.

- Students will be able to interpret data obtained from research articles.

- Students will be able to work in research group and reflect on their work.

Ingangseisen

To start the minor student has to have minimum of 75 EC related to Life Sciences & Technology and successfully completed courses of Molecular Biology (or similar courses involving topics of cell, biochemistry, molecular biology), including the basic skills in  molecular cloning. Foreign students are asked to deliver the proof of successfully completed course of safety work in biology/microbiology laboratories.

The documents proving the student eligibility must be send to email martina.sura@hvhl.nl in electronical version: Overview of current status of obtained ECs together with the list of courses from which the ECs were obtained.

Literatuur

No mandatory literature

Recommended literature:

Thomas A., Thrive in Genetics, Oxford University Press, 2013, ISBN: 9780199694624

Primrose S.B. and Twyman R.M., Principles in Gene Manipulation and Genomics, John Wiley And Sons Ltd, 2006, seventh edition, ISBN: 9781405135443

Rooster

Students get 30 EC when successfully finalizing the course.  Course consists of six parts (A, B, C, D, E, F):

 

Part A (Theory): 160 hours

Lectures 46 hours

Self-study 110 hours 

Exams 4 hours

 

Part B (Practical instructions in the laboratory): 88 hours

Preparation/self-study 32 hours 

Practical instruction 32 hours 

Presentation 24 hours

 

Part C (Project design/MTA): 166 hours

Literature study 80 hours 

Tutor meetings 10 hours

Research proposal 60 hours

Clone manager practicums 16 hours

 

Part D (Laboratory assignment/ETA): 290 hours

Self-study/preparation 97 hours

Tutor meetings 15 hours

Practical work in the lab 112 hours

Work discussions/presentations 46 hours

Poster presentation 24

 

Part E (-Omics Journal club): 108 hours

Self-study/articles analysis 61 hours 

Work discussions 27 hours

Presentations 8 hours

Lectures 12 hours

 

Part F (Self-reflection): 28 hours

Preparation 22 hours

Report 6 hours

Toetsing

Students are graded based on six assessments.

 

Assessment A: Theory knowledge is assessed by written exam (open and multiple choice questions) – individual grading. Grading 0.1 – 10.

 

Assessment B: The practical instruction in the laboratory are assessed by the attendance (mandatory), commitment, lab journal and a presentation (sufficient/insufficient) – group grading.

 

Assessment C: The project/research design for cloning experiment (for laboratory assignment) is assessed by written Research proposal, presentation and midterm assessment (MTA) and attendance and active participation during realization of the Research proposal – individual grading, grade 0.1-10.

 

Assessment D: The practical performance of the course assignment is assessed by the attendance and active participation in the laboratory, at tutor meetings and work discussions (minimum 80%). Data interpretation is assessed by poster presentation and end term assessment (ETA) (grade 0.1 – 10) – individual grading.

 

Assessment E: The Journal club is assessed by the attendance, article analysis and presentations (mandatory) (sufficient/insufficient) – individual grading.

 

Assessment F: An important feature of an HBO-skilled biotechnology analyst is the ability of self-reflection, even more when we work as a team. The student writes a reflection on his/her function/role in the minor Bioengineering and  -omics. Individual grading, assessed as sufficient/insufficient.

Aanvullende informatie

Target group:

This course is suitable for motivated students of biotechnology, biomedical sciences and forensic sciences having an interest in learning latest techniques of molecular cloning (CRISPR Cas9) and in learning a holistic understanding of the CRISPR landscape and its future potential.

Students have to have successfully completed basic courses either in Molecular Biology, Molecular Detection, Molecular Medicine or similar courses involving topics of cell, biochemistry, molecular biology and basic cloning techniques.